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Analytical ultracentrifugation (AUC) is a fundamental and powerful tool for studying the size and shape of macromolecules in solution. It is particularly important for characterizing protein aggregation. Alliance Protein Laboratories has been offering AUC analysis services (both sedimentation velocity and sedimentation equilibrium) since 1999. We were the first contract lab in the US to offer this service, and to our knowledge still the only one with our own instruments in-house. APL has completed hundreds of AUC projects on proteins, peptides, nucleic acids, and other molecules. At A.P.L. all the AUC experiments and interpretation are done by John Philo, a recognized AUC expert who has 18 years experience in biotech product development, who pioneered new AUC software methods for analyzing biotech products and their interactions, and who has over 40 publications involving AUC measurements. The sedimentation velocity and sedimentation equilibrium pages give further background about, and examples of, these two primary AUC techniques. A.P.L. has two analytical ultracentrifuges: a Beckman-Coulter XL-I equipped with both absorbance and Rayleigh interference (refractive index) detection (picture) and also a Beckman-Coulter XL-A (absorbance data only). Turn-around for AUC analysis is typically 2-4 weeks. Before even considering using another company for AUC analysis see questions you need to ask a biophysical lab. Below are downloadable versions of a few of our presentations and publications about AUC (more can be found on the Further Reading page):
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