Further examples of applications of our biophysical techniques can be
obtained through links below to Adobe Acrobat .pdf format versions of posters and talks we have given at various industry or scientific meetings,
our review article about SEC-MALLS, and other published
articles. These are listed in reverse chronological order.
"Aggregation
analysis of therapeutic proteins, part 3". Arakawa, T., Philo, J. S., Ejima, D.,
Sato, H., and Tsumoto, K. (2007). Bioprocess International 5 (10), 52-70.
"Focus
on aggregation: types, causes, characterization, and impact", invited talk,
Comparability for Biotherapeutics, Berlin June 2007
"Aggregation
analysis of therapeutic proteins, part 2". Arakawa, T., Philo, J. S., Ejima, D., Tsumoto,
K., and Arisaka, F. (2007). Bioprocess International 5 (4), 36-47.
"Aggregation analysis of
therapeutic proteins, part 1". Arakawa, T., Philo, J. S., Ejima, D., Tsumoto,
K., and Arisaka, F. (2006). Bioprocess International 4 (10), 42-49.
Monitoring the homogeneity of adenovirus preparations (a gene therapy
delivery system) using analytical ultracentrifugation. Berkowitz, S. A. and
Philo, J. S. (2007). Anal. Biochem.
362, 16-37. [PDF available only via e-mail request].
"Is Any Measurement Method Optimal for All
Aggregate Sizes and Types?", Philo, J.S. (2006) AAPS Journal. 8(3): E564-E571
"Analyzing Aggregates by Sedimentation
Velocity and Light Scattering", invited talk, AAPS National Biotechnology
Meeting, 2006 [484 KB]
"Some Unusual Aggregation Phenomena in
Recombinant Proteins", poster, WCBP 2006 [160 KB]
"Sensitivity and Reproducibility of Protein
Aggregate Analysis by Sedimentation Velocity", poster, WCBP 2005 [316 KB]
"Analyzing Aggregates of Different Sizes
and Types: SEC vs. Analytical Ultracentrifugation vs. Light
Scattering", invited talks, WCBP and IBC Well Characterized Biologicals, 2004 [793 KB]
"Measuring Comparability of Conformation,
Heterogeneity, and Aggregation with Circular Dichroism and Analytical
Ultracentrifugation", invited talk, State of the Art Methods for the Characterization of
Biological Products and Assessment of Comparability, NIH, June 2003 [682 KB]
"Characterizing
the aggregation and conformation of protein therapeutics", Philo, J. S.
(2003), Amer. Biotech. Lab. 21, 22-26.
"Limitations and Advantages in Assessing
Adenovirus Homogeneity by Laser Light Scattering and Analytical
Ultracentrifugation - Measuring the Aggregation of Large Biopharmaceuticals",
invited talk, Steve Berkowitz (Biogen-Idec) and John Philo, WCBP 2003 [514 KB]
"Some Biophysical Tools for
Demonstrating Comparability of Conformation and Aggregation",
poster, WCBP 2002
[235 KB]
"Kinetic and
Thermodynamic Analysis of Thermal Unfolding of Recombinant Erythropoietin", Biosci.
Biotechnol. Biochem. 65, 121-1327, 2001 [1.1 MB]
"Online Size-Exclusion High-Performance
Liquid Chromatography Light Scattering and Differential Refractometry Methods to
Determine Degree of Polymer Conjugation to Proteins and Protein-Protein or
Protein-Ligand Association States", B.S. Kendrick, B.S. Kerwin, B.
Chang, and J.S. Philo (2001) Anal. Biochem. 299, 136-146 [109 KB]
"New Software Methods Enhance
Sedimentation Velocity Analysis of Protein Aggregation and Conformation",
poster, WCBP 2001 [297 KB]
"Analysis of Aggregation, Stability,
and Lot Comparability by Sedimentation Velocity", poster, WCBP 2000 [352 KB]
"Effects of Additives on
Reversibility of Thermal Unfolding", poster, WCBP 2000 [223 KB]
"Size-Exclusion Chromatography with On-Line
Light-Scattering, Absorbance, and Refractive Index Detectors for Studying
Proteins and Their Interactions" (1996 review article)
[236 KB]
