Alliance Protein Laboratories specializes in biophysical and biochemical characterization of proteins, nucleic acids, and other macromolecular assemblies (viral vectors for gene therapy, protein-polymer conjugates, etc.) We offer characterization services not available in-house from any other U.S. contract lab, including analytical ultracentrifugation (AUC) and concentration-gradient multi-angle light scattering (CG-MALS). Our scientists pioneered the application of a number of these biophysical techniques in the biotechnology industry.
APL offers various techniques to characterize or compare protein higher order structure (HOS). These include methods to characterize protein folding (secondary/tertiary structure), protein stability, protein conformation, protein aggregation, and the oligomeric state of the native protein (quaternary structure).
For characterization of oligomeric state and aggregation, APL offers analytical ultracentrifugation (AUC), light scattering, and native gel electrophoresis analysis services. For characterization of protein conformation and stability we, primarily use circular dichroism (CD), differential scanning calorimetry (DSC), and fluorescence, but native gels are also useful.
APL can also quantify protein-protein or protein-ligand interactions, e.g. for functional assessment of antibody-antigen or hormone-receptor pairs using analytical ultracentrifugation.
All the techniques offered and some of their uses are listed on the Methods Summary page. Several general applications and the benefits of detailed characterization are discussed on the Example Applications page. More details about the background and applications for some of the techniques are discussed on their individual pages:
KBI Biopharma Provides
Our parent company KBI Biopharma is a global biopharma contract development & manufacturing organization driven to accelerate the development of innovative discoveries into life-changing biological products and expand global access of medicines to patients in need. To date, they’ve served over 250 clients around the world including most of the top ten pharmaceutical companies.
Key Benefits from Using APL for Characterization Services
Outsourcing to APL gives you access to equipment, methods, and expertise that few companies have in-house
Data you can trust, provided by widely-published scientists who are recognized experts and who have extensive experience in pharmaceutical applications
Free guidance about what methods are best to address your characterization needs or issues with your product (and if we don't think we can help you, we'll say so!)
At APL we don't just run samples, we work collaboratively with our clients
We want to be sure our clients understand the data we provide and are able to present the results to their colleagues and managers in a meaningful way that will influence decision-making
Our reports explain the data and provide necessary background so your scientists can really use the results
If needed, we can present the data at your site and can contribute our expertise as consultants to interpreting its implications for the development of your product